Dynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Wounds

Background. Management of the reparative process is an urgent task of modern medicine. In our opinion, the development of pathogenetically grounded approaches to optimizing the repair process for managing the interrelations of stromal cells is promising. One of the promising areas in this regard is the impact on the MAPK-cascades.Aim: to study the expression of MAP-kinase mechanisms in the regulation of repair by the example of a musculocutaneous wound.Methods. A linear muscular skin wound was modeled using Wistar rats weighing 220–250 g at the age of 9 months (n = 24). Immunofluorescence staining was performed to detect the activity of p38, JNK, and ERK MAPK cascades from 1 to 30 days.Results. It was established that specific staining in the area of connective tissue formation during staining with p38 MAPK and its phosphorylated form was first observed on the 3rd day, and its maximum severity occurred at the same time. On the 7th and 14th day, small zones in the area of scar formation were minimally stained. The phosphorylated part of the JNK-cascade in the zone of traumatic injury was detected starting from the 1st day after the injury. Bright color persisted on the 3rd day. On the 7th day, the color was minimal, and by the 14th day a second wave of expression was observed. ERK-staining was observed from the 1st to the 14th day with a peak activity on the 3rd day.Conclusion. Thus, we revealed the simultaneous involvement of p38, JNK-, and ERK-cascades in the regulation of the reparative process in the conditions of a musculoskeletal wound. At the same time, it is noteworthy that the peak activity of all cascades coincides and falls on the 3rd day

Truncated estimating parameters of additive mixture of radio signal and kurtosis non-Gaussian noise

Побудовано ефективні методи статистичної обробки даних відповідного класу, які б дозволили підвищити точність результатів та швидкість обробки сигналів Синтезовано алгоритми знаходження спільної оцінки частоти радіосигналу при усіченому оцінюванні дисперсії негаусівської ексцесної завади. Запропоновані моделі та алгоритми можуть бути застосовані до різного класу сигналів і завад, а отримані результати – використані для підвищення точності оцінок параметрів сигналів в радіолокації, радіонавігації, телекомунікації та інших сферах, де точність алгоритмів обробки сигналів відіграє важливу роль.Introduction. Classical method based on the use of Gaussian random signal model has its advantages and disadvantages. Therefore, Maximum Likelihood Method has not found wide implementation due to the high computational complexity. Method of Moments does not have the properties of asymptotic optimality, although it leads to a relatively simple calculations. In general, the methods do not consider more complex structure of real noise. Therefore the accuracy of signal processing algorithms may be insufficient. The aims and objectives of research. The aim of the paper is to adapt Methods of Polynomial Maximization (MPM) and Truncated Stochastic Polynomial Maximization (MTSPM) for joint estimation of radiosignal and kurtosis non-Gaussian noise parameters. The Objective of research is to develop effective methods of statistical data processing, which would allow increasing the accuracy and speed of signal processing. Construction of the Polynomial algorithms for joint estimating. The systems of equations are constructed to find joint estimates. MPM is used to estimate the radiosignal frequency and the noise variance – MTSPM. Statistical properties of the radiosignal frequency estimates. The asymptotic dispersions of estimates are calculated to study the statistical properties of radiosignal parameter estimates. Comparison of the asymptotic dispersion of radiosignal frequency estimates and a graphical representation of the results. The efficiency of polynomial estimation algorithms increases with the stochastic polynomial degree and as the values of coefficients of kurtosis approach the tolerance range limit. Conclusion. The effective methods of signal processing to enhance the accuracy and speed of non-Gaussian signals processing are developed. The results can be used to improve the estimation accuracy of radiosignal parameters in radiolocation, radio navigation and other areas, where the accuracy of signal processing algorithms plays an important role.Построено методы статистической обработки данных соответствующего класса, которые бы позволили повысить точность результатов и скорость обработки сигналов. Синтезированы алгоритмы нахождения совместной оценки частоты радиосигнала при усеченном оценивании дисперсии эксцессной помехи. Предложенные модели и алгоритмы могут быть применены к разного класса сигналам и помехам, а полученные результаты – использованы для повышения точности оценок параметров сигналов в радиолокации, радионавигации, телекоммуникации и других сферах, где точность алгоритмов обработки сигналов играет важную роль

Використання поліфенолкарбонового комплексу з антарктичних чорних дріжджів Nadsoniella nigra в раціон птиці та їх вплив на інкубаційні якос-ті яєць перепілок несучок

The results of research on the influence of the polyphenol carbon complex from the Antarctic black yeast Nadsoniella nigra, which is additionally introduced into compound feed, are presented on the hatching qualities of quail eggs. Experimental studies were conducted in the conditions of the problematic research laboratory of feed additives of the National University of Bioresources and Nature Management of Ukraine. The material for the scientific and economic experiment was adult quail of the Pharaoh breed. Experiments were conducted using the group method. Accordingly, 192 heads of quails were selected for the experiment at 42 days, from which four groups were formed according to the principle of analogs - a control group and three experimental ones, 48 heads in each with four replications). When selecting analogs, the sex, age, and body weight of the bird were taken into account. The main period lasting 210 days, was divided into seven sub-periods, each of which lasted 30 days. The composition of the compound feed for the quails of the control and experimental groups and the set and number of ingredients in it were identical. The chemical composition of the combined feed used for feeding quails was the same and differed only in the content of PFC. During the experiment, the preservation of livestock and productivity were recorded, and feed conversion was also calculated. It was experimentally established that adding 0.1, 0.5, and 1.0 mg/kg polyphenol-carbon complex from the Antarctic black yeast Nadsoniella nigra to compound feed positively affects the hatching properties of eggs and, at the same time, reduces the percentage of hatching waste. At the same time, in the birds of the 3rd experimental group, which were fed 0.5 mg/kg of PFC as part of the compound feed, respectively, the percentage of fertilization was 2.2%; hatchability of eggs by 3.8%; and the yield of young by 5%, compared to birds that were not given PFC compound feed.Наведено результати досліджень з установлення впливу поліфенолкарбонового комплексу з антарктичних чорних дріжджів Nadsoniella nigra, який додатково вводять у комбікорми, на інкубаційні якості яєць перепелів. Експериментальні дослідження проводилися в умовах проблемної науково-дослідної лабораторії кормових добавок Національного університету біоресурсів і природокористування України. Матеріалом для науково-господарського досліду були дорослі перепели породи фараон. Досліди проводили за методом груп. Відповідно до цього для досліду у 42-добовому віці було відібрано 192 голови перепелів, з яких за принципом аналогів сформовано 4 групи – контрольну і три дослідні, по 48 голів у кожній з чотирма реплікаціями). При підборі аналогів враховували стать, вік і масу тіла птиці. Основний період тривалістю 210 діб було поділено на 7 підперіодів, кожен із яких тривав 30 діб. Склад комбікорму для перепелів контрольної та дослідних груп, набір і кількість інгредієнтів у ньому були ідентичними. Хімічний склад комбікорму, який використовували для годівлі перепелів, був однаковим і різнився лише за вмістом ПФК. Упродовж досліду проводився облік збереженості поголів’я, продуктивності, а також розраховували конверсію корму. Експериментально встановлено, що додавання до комбікорму 0,1, 0,5 та 1,0 мг/кг поліфенолкарбонового комплексу з антарктичних чорних дріжджів Nadsoniella nigra позитивно впливає на інкубаційні властивості яєць і при цьому знижує відсоток відходів інкубації. Водночас у птиці 3-ї дослідної групи, якій у складі комбікорму згодовували 0,5 мг/кг ПФК, відповідно за відсотком запліднення на 2,2 %; виводимістю яєць на 3,8 %; та виходом молодняку на 5 %, порівняно з птицею, якій не вводили до складу комбікорму ПФК

Use of Vasavital® in patients with diabetic retinopathy

Background: Diabetic retinopathy (DR) is a major cause of visual impairment or blindness among working-age adults worldwide. For years, researchers around the world have been trying to develop new effective pharmaceutical methods of treatment for preclinical and early DR. Purpose: To examine the effect of a one-month course of Vasavital on the function of the visual system and ocular hemodynamics (using ophthalmic rheography) in patients with non-proliferative and proliferative diabetic retinopathy (NPDR and PPDR, respectively). Material and Methods: Forty-seven type 2 diabetes patients with DR and moderate glycemic control were divided into those with NPDR (group 1 of 15 patients; 30 eyes) and those with PPDR (group 2 of 17 patients; 34 eyes). The control group was composed of 15 volunteers (30 eyes) of similar age having no systemic or eye disease. Patients received a one-month course of Vasavital-only therapy at a dose of one capsule twice a day as an outpatient treatment. They received visual acuity assessment, intraocular pressure measurement, ophthalmoscopy, biomicroscopy, perimetry, systemic blood pressure and pulse measurement, optical coherence tomography and fluorescein angiography, and ocular hemodynamics was assessed by ophthalmic rheography. Eleven patients (22 eyes) with NPDR and ten patients (20 eyes) with PPDR underwent electrophysiological studies of electrically evoked phosphene threshold (EEPT) and critical frequency of phosphene disappearance (CFPD), before and after a course of Vasavital treatment. Results: Patients reported that a one-month course of Vasavital was well-tolerated, with no new complaints. In addition, no side effects were observed. After treatment, the function of the photopic afferent system as assessed by light sensitivity at minutes 0 to 7 of adaptation improved by 33.3%-40% in patients with NPDR and by 27.2%-33.3% in patients with PPDR. In addition, there was a decrease in EEPT by 18% and 7.7%, respectively, and an increase in CFPD by 28.2% and 24.7%, respectively, for patients in groups 1 and 2. Moreover, ocular pulse blood filling improved by 27.7% in patients with NPDR and by 17.3% in patients with PPDR, and vascular tone in large-caliber vessels decreased by 8% in the former patients. Conclusion: A one-month Vasavital course administered to patients with DR had a positive effect on the visual system function and ocular circulation parameters, which provides grounds for the use of the Ginkgo biloba-based preparation as a monotherapy or as part of a combined treatment for initial functional changes in the visual system in DR

Biodiversity surveys of grassland and coastal habitats in 2021 as a documentation of pre-war status in southern Ukraine

Background This paper presents two sampling-event datasets with occurrences of vascular plants, bryophytes and lichens collected in May-June 2021 in southern Ukraine. We aimed to collect high-quality biodiversity data in an understudied region and contribute it to international databases and networks. The study was carried out during the 15th Eurasian Dry Grassland Group (EDGG) Field Workshop in southern Ukraine and the Dark Diversity Network (DarkDivNet) sampling in the Kamianska Sich National Nature Park. By chance, these datasets were collected shortly before the major escalation of the Russian invasion in Ukraine. Surveyed areas in Kherson and Mykolaiv Regions, including established monitoring plots, were severely affected by military actions in 2022. Therefore, collected data are of significant value in the context of biodiversity documentation. The knowledge about the biodiversity of this area will help to assess the environmental impact of the war and plan restoration of the damaged or destroyed habitats. The first preliminary analysis of collected data demonstrates the biodiversity richness and conservation value of studied grassland habitats. New information We provide sampling-event datasets with 7467 occurrences, which represent 708 taxa (vascular plants, bryophytes and lichens) collected in 275 vegetation relevés. Amongst them, vascular plants are represented by 6665 occurrences (610 taxa), lichens - 420 (46) and bryophytes - 381 (51). Several new species were reported for the first time at the national or regional level. In particular, one vascular plant species (Torilis pseudonodosa) and two lichen species (Cladonia conista, Endocarpon loscosii) were new to Ukraine. One vascular plant (Stipa tirsa), two species of bryophytes (Rhynchostegium megapolitanum, Ptychostomum torquescens) and three species of lichens (Cladonia cervicornis, C. symphycarpa, Involucropyrenium breussi) were recorded for the first time for the Kherson Region. Additionally, these datasets contain occurrences of taxa with narrow distribution, specialists of rare habitat types and, therefore, represented by a low number of occurrences in relevant biodiversity databases and particularly in GBIF. This publication highlights the diversity of natural vegetation and its flora in southern Ukraine and raises conservation concerns

Antrax in Primorsky Territory (1919–2020). Communication 2. Episootiological- Epidemiological Situation and Zoning of Administrative Territories

The aim of the study was to analyze the epizootiological and epidemiological situation in the Primorsky Territory (1919–2020) and to zone the administrative districts in regard to anthrax. Materials and methods. Accounting and reporting documents, information and archival materials were collected and analyzed, statistical reference books and literature sources were used. A comprehensive epizootiological and epidemiological survey of 12 supposed places of anthrax burials and cattle burial grounds was carried out, and their biological hazard assessed. Results and discussion. Within the framework of updating the Cadastre of stationary potentially hazardous as regards anthrax areas in the Russian Federation (2005), the number of infected farm animals and human cases was specified: according to official data, over the period of 1930–1979, the disease in 173 animals and 34 people with five deaths was registered in 82 stationary unfavorable for anthrax areas of 22 municipalities of the region. Together with the veterinary service of the region, an audit of 12 supposed places of anthrax burials in seven districts of the region was conducted. Based on the results of a comprehensive epizootiological and epidemiological survey, high biological hazard was established for 2 and potential one – for 1 anthrax burials. The culture of Bacillus anthracis isolated on the territory of Primorsky Territory belongs to one of the canonical SNP clusters – A.Br.008/009 of the global genetic line A (subgroup A1). Five criteria for assessing the epizootiological and epidemiological disadvantage for anthrax and the ranking of indicators of the administrative territories in the region have been determined. Zoning of the administrative territories of the region according to risk of anthrax has been carried out. Prophylactic anti-epizootic and anti-epidemic measures have been put forward

Making tourist guidance systems more intelligent, adaptive and personalised using crowd sourced movement data

Ambient intelligence (AmI) provides adaptive, personalized, intelligent, ubiquitous and interactive services to wide range of users. AmI can have a variety of applications, including smart shops, health care, smart home, assisted living, and location-based services. Tourist guidance is one of the applications where AmI can have a great contribution to the quality of the service, as the tourists, who may not be very familiar with the visiting site, need a location-aware, ubiquitous, personalised and informative service. Such services should be able to understand the preferences of the users without requiring the users to specify them, predict their interests, and provide relevant and tailored services in the most appropriate way, including audio, visual, and haptic. This paper shows the use of crowd sourced trajectory data in the detection of points of interests and providing ambient tourist guidance based on the patterns recognised over such data

Beliefs and preferences regarding biological treatments for severe asthma

Background: Severe asthma is a serious condition with a significant burden on patients' morbidity, mortality, and quality of life. Some biological therapies targeting the IgE and interleukin-5 (IL5) mediated pathways are now available. Due to the lack of direct comparison studies, the choice of which medication to use varies. We aimed to explore the beliefs and practices in the use of biological therapies in severe asthma, hypothesizing that differences will occur depending on the prescribers’ specialty and experience. Methods: We conducted an online survey composed of 35 questions in English. The survey was circulated via the INterasma Scientific Network (INESNET) platform as well as through social media. Responses from allergists and pulmonologists, both those with experience of prescribing omalizumab with (OMA/IL5) and without (OMA) experience with anti-IL5 drugs, were compared. Results: Two hundred eighty-five (285) valid questionnaires from 37 countries were analyzed. Seventy-on percent (71%) of respondents prescribed biologics instead of oral glucocorticoids and believed that their side effects are inferior to those of Prednisone 5 mg daily. Agreement with ATS/ERS guidelines for identifying severe asthma patients was less than 50%. Specifically, significant differences were found comparing responses between allergists and pulmonologists (Chi-square test, p < 0.05) and between OMA/IL5 and OMA groups (p < 0.05). Conclusions: Uncertainties and inconsistencies regarding the use of biological medications have been shown. The accuracy of prescribers to correctly identify asthma severity, according to guidelines criteria, is quite poor. Although a substantial majority of prescribers believe that biological drugs are safer than low dose long-term treatment with oral steroids, and that they must be used instead of oral steroids, every effort should be made to further increase awareness. Efficacy as disease modifiers, biomarkers for selecting responsive patients, timing for outcomes evaluation, and checks need to be addressed by further research. Practices and beliefs regarding the use of asthma biologics differ between the prescriber's specialty and experience; however, the latter seems more significant in determining beliefs and behavior. Tailored educational measures are needed to ensure research results are better integrated in daily practice

7th Drug hypersensitivity meeting: part two

No abstract availabl